| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McDonald, Logan J (12)
|
NCTYDE |
35.51 |
34.56 |
-0.95 |
| 2 |
Valliere, Alexander H (12)
|
NCTYDE |
37.61 |
36.67 |
-0.94 |
| 3 |
Greene, Jonah R (12)
|
NCTYDE |
41.10 |
38.16 |
-2.94 |
| 4 |
Matilainen, Aaron E (12)
|
NCTYDE |
42.08 |
40.44 |
-1.64 |
| 6 |
Hulme, Evan R (11)
|
NCTYDE |
43.66 |
43.16 |
-0.50 |
| 7 |
Maxey, Stone H (11)
|
NCTYDE |
47.09 |
43.90 |
-3.19 |
| 10 |
Danhauer, Hayden T (11)
|
NCTYDE |
51.49 |
45.22 |
-6.27 |
| 11 |
Reboussin, Auguste M (12)
|
NCTYDE |
53.44 |
45.81 |
-7.63 |
| 14 |
Eldeeb, Ahmed K (11)
|
NCTYDE |
51.25 |
49.96 |
-1.29 |
| 16 |
Riley, Connor A (12)
|
NCTYDE |
|
51.15 |
- |
|
Halicigil, Baran (11)
|
NCTYDE |
48.91 |
DQ |
- |